CONTINUOUS IN VITRO CULTIVATION OF SPHERULES OF COCCIDIOIDES IMMITIS
نویسندگان
چکیده
منابع مشابه
Continuous in Vitro Cultivation of Spherules of Coccidioides Immitis.
Breslau, Abraham M. (Veterans Administration Center and University of California Los Angeles Center for the Health Sciences, Los Angeles, Calif.), and Mike Y. Kubota. Continuous in vitro cultivation of spherules of Coccidioides immitis. J. Bacteriol. 87:468-472. 1964.-Spherules of Coccidioides immitis were grown in vitro in continuous culture for more than 4 years. Cultures were grown in modifi...
متن کاملSerological comparison of spherules and arthrospores of Coccidioides immitis.
Spherule and arthrospore cellular preparations were sonic-treated and separated into their respective supernatant and sediment components. Complement-fixation tests with antispherule and antiarthrospore pooled rabbit sera revealed that the soluble antigens exhibited more serological activity than the sediment preparations. After autoclaving, an arthrospore cellular antigen exhibited increased a...
متن کاملElectron Microscopy of Cultured Spherules of Coccidioides Immitis
Spherules of C. immitis have been grown in vitro in modified Roessler's medium under CO(2) tension and continuous cultures now maintained for over 18 months. Transformation of hyphae and development of the spherule form have been studied by thin section electron microscopy. Cells of organisms in the hyphal stage have thin (ca. 50 mmicro), apparently structureless walls and a cytoplasmic membran...
متن کاملEffects of lysozyme and chitinase on the spherules of Coccidioides immitis in vitro.
Spherules of Coccidioides immitis strain Silveira produced in vitro were treated with chitinase and lysozyme. The walls of merthiolate-killed mature endosporulating spherules were degraded by chitinase (500 mug/ml) and by lysozyme (100 and 500 mug/ml). Thus, as was visible through the light microscope, the spherule wall was reduced in thickness from 1 to 2 mum to less than 0.5 mum. The degradat...
متن کاملIn vitro production of the spherule phase of Coccidioides immitis.
The in vitro growth of the spherule (sporangium) phase of Coccidioides immitis has posed a problem of prime interest in the study of the disease coccidioidomycosis. The fungus is dimorphic in that it has a cultural, or saprophytic, phase in which growth consists of mycelium and arthrospores, and a tissue or parasitic phase consisting of thick-walled nonbudding spherules (sporangia) varying in s...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1964
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.87.2.468-472.1964